This paper circulates around the core theme of a) There exists a gene in this sequence, use the translate tool at http://au.expasy.org/tools/dna.html to find the most likely protein product. together with its essential aspects. It has been reviewed and purchased by the majority of students thus, this paper is rated 4.8 out of 5 points by the students. In addition to this, the price of this paper commences from £ 99. To get this paper written from the scratch, order this assignment now. 100% confidential, 100% plagiarism-free.
a) There exists a gene in this sequence, use the translate tool at http://au.expasy.org/tools/dna.html to find the most likely protein product.
i. Paste the protein sequence into your assignment and indicate which frame you used. ii. Why are there 6 possible frames? Hint: the longest sequence Met -> Stop will be the correct outcome.
b) Using the translator you have found the start and end of the gene, trim the nucleotide sequence to show just the coding sequence i. How many nucleotides where removed from the front or end of the above sequence?
c) Use the Nucleotide sequence, transcribe the first 21 nucleotides into RNA, and then translate that into amino acids. d) Paste the nucleotide sequence of the whole gene into NCBI Blast and perform a BLASTX search against SwissProt database.
i. What organism was my sequence most closely matching? ii. Give a brief summary on the function of the closely matching protein?
2) Biological Sequence alignment. (5 marks)
a) Calculate the alignment score for different alignments and indicate the best possible aligned sequence for the following sequence:
ACCTAGCTAGCCGAT ACCCCTAGGCGAAA Use Match: +1, Mismatch:-1 and Indel: -2
i. Show five possible alignments and their scores ii. Which is the best possible alignment? State the reason.
b) Carry out a Multiple Sequence Alignment (MSA) for the protein Ras 1. Use the UniProt (http://www.uniprot.org/) database for retrieving the protein sequence of keratin type2. The protein sequences from the following organisms must be included in your MSA. 1) Homo sapiens (Human) 2) Drosophila melanogaster (Fruit Fly) 3) Candida albicans (Yeast) 4) Mus musculus (Mouse) 5) Gallus gallus (Chicken)
