A batch-specific calibration factor applies to each kit made by the manufacturer and is found on
the kit specification sheet.
1. Calculate the mean absorbance of the duplicates of the cut-off Calibrator and multiply by the
calibration factor. This is the Cut-off Value.
2. Calculate the mean absorbances of each patient sera.
3. Calculate the Index Value for each patient by dividing the mean sample absorbance by the
Cut-off Value.

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